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National grid ma login gas3/21/2024 If you smell gas on the street or in your home, please call 9-1-1 from a safe location outside your home! You may also call the National Grid hotline at 1-80 to report a possible gas leak. Neighbors in these areas should have received notifications from National Grid.įor questions about the Weston Road gas main project, please contact National Grid at 78. Registering takes just five minutes and turns time into savings. Register for online access to your account and use our portal to view your bill, set up automatic payments, receive a personalized Energy Savings Plan, and more. The specific timeline for this project is subject to change due to weather and permitting. Gas Emergencies Power Outages Contact Us. Phase 3: Weston Road and Route 9 interchange (Work will be done overnight).Phase 2: Weston Road North of Route 9 between Elmwood Road and Cleveland Road.Phase 1: Weston Road North of Route 9 between Elmwood Road and Pine Plain Road.Sign in with your email address or register for a new account today. You can choose from various payment options, view your bill history, and manage your account preferences. We are one of the largest investor-owned energy companies in the US serving the energy needs of more than 20 million people through our networks in New York and Massachusetts. National Grid - Bill PayPay your National Grid bills online with ease and convenience. At times, Weston Road will be limited to one lane and drivers will be detoured to side streets.ĭrivers are strongly encouraged to find alternate routes during this project. National Grid is an electricity, natural gas, and clean energy delivery company, where our 17,000 employees live and work in the communities we serve. This work will take place Monday through Friday during daytime hours from 7:00 a.m. Who has this information on hand each time? I have to open my bills each time to get this information! Why bother? Who knows this on a whim? This is my most disliked app on my phone.National Grid is replacing gas mains along portions of Weston Road and side streets in the coming months. It should be easy to switch between gas/electric bills since they are both national grid but I have to log out and log in with the different account numbers each time. I do not have a choice but to use this app unless I want to waste more time on the phone. National Grid is one of the world’s largest publicly listed utilities focused on transmission and distribution of electricity and gas. It does NOT make your life easier like apps should to pay bills. If you smell gas anywhere including in your home, report it here immediately. Did you know natural gas is naturally tasteless, colorless, and odorless We add mercaptan, a sulfur-smelling chemical, to our natural gas to help you recognize a leak immediately. It’s just as a whole VERY frustrating to use this app. Assistance is available 24 hours a day, every day. Face recognition option would be nice so I don’t have to remember a password each time when things aren’t working. Follow us for speedy customer service, news, and updates. Send account questions, streetlight outage or energy theft reports, and/or feedback. Report a Gas Emergency Carbon Monoxide Odor Fade. I appreciate the security to get to my account but no one is going to pay my bills but me. Click to expand the menu for Natural Gas Safety. ![]() ![]() If you are moving your home or business, it’s easy to transfer your services with us. I created a profile to save my card information but logging in and paying a bill takes just as long as if I called in. It’s cleaner, more affordable, and better for the environment. Then suddenly one of them kept giving me an error so I switched to the app thinking it’d be easier than calling and having to give my card info over the phone each month. I was given emailed bills for gas/electric each month which I could very easily pay through.
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Compressing video files online free3/21/2024 ![]() The size of the bit rate is directly proportional to the video file size. Bit rate (data rate): Bit rate is the amount of data that is used by each video file, and it is generally measured in kilobits per second.Various factors affect the size of a video file: Larger the file size, the more significant the area needed. The video size also decides the amount of space it occupies on your PC storage. Moreover, many social networking and video sharing sites have limitations to the size of the video that can be uploaded to their website. The size of the video file is essential as it determines the time and data needed for downloading as well as uploading. There are many different types of video file formats available, but all the files feature characteristics, including – a container type, video signal, audio signal, and a codec. When it comes to video files, they are generally large, as they need large data to depict the video signal accurately. Choose which method to compress videos according to your needs.Ī file format defines the structure in which file information is stored. Types of video compression, including lossless video compression and lossy video compression, the lossless compression always keep a higher video quality after compressed while the lossy compression lowers the quality. ![]() The video size can be affected by many parameters, including video resolution, video format, codec, bit rate, and others. The video compression definition simply refers to the process of reducing a video size by encoding the video. To make your video file compression task easy, read below to know the basics of video file size, factors affecting its size, and related details. Knowledge About Video Compression You Should Know A Comparison Table for You to Select the Proper Video Compressor ![]() Top 11 Free Video Compressor to Compress Video Online ![]() How to Compress Video Files Without Losing Quality on Windows/Mac Top 13 Video Compressors Online/Windows/Mac/iOS/Android Are you looking for ways on how to compress a video, like compressing MP4, MOV, MKV, even DVD movies? We have the best video compression solutions listed in the parts below. Considering the above benefits, reducing the file size seems to be of utmost importance. Moreover, compressed videos consume fewer Internet data while uploading, and the overall process is quick. Video compression also becomes necessary when you want to upload your video to a site where there is a limitation to the size that can be uploaded. Do not fret as you can compress video and keep the past and add the new ones as well. Delete some old favorites to accommodate a few new ones, but this is only a temporary solution. So now, when you have a massive selection of videos that surpasses your PC storage, you may get the same questions listed above. If you are a movie buff, you know the research, time, and patience that are needed to create a collection of your favorite videos over the years. 5.25 11 Ways to Compress Videos Free Online.4.5 Compress Video for Facebook, Instagram.3.10 HandBrake Video Compressor AlternativeĤ.3.5 Compress Videos with Windows Media Player Alternatives.3.3 Make Windows Movie Maker Resize Video.2.10 Top 8 Video Size Reducers for Android.2.9 10 Video Compressor App Android/iPhone.2.7 MMS Video Size Limit for iPhone/Android.Compress Video/Audio in Different Formats +
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Grim reaper tattoo stencil3/21/2024 Mix and match this set with other Tattoo Pro airbrush stencil sets to create something unique every time!īecause of the size of these stencils, it is easiest to use them with an airbrush versus sponging the designs on. These stencils are perfect for all ages, and with a little practice you can amaze your clients with these killer designs! The Grim Reaper stencil will definitely be a hit for those looking for cool and creepy designs. He wanted to create stencils that really looked like authentic tattoos so that airbrush artists could paint full sleeves, back pieces, chest pieces and so on. See more ideas about grim reaper, reaper tattoo, grim reaper art. Tattoo Pro stencils were the brain child of graffiti and body painting artist, Wiser Oner. These sketches are great, Cain, Domino tells me as he flips through the pages that consist of a few dragons, a grim reaper. Explore Mike Hansulds board 'Grim reaper', followed by 322 people on Pinterest. ![]() We do not endorse the actions of or rhetoric of all the people included in these collections, but we think they are important for growing minds to learn about under the guidance of parents or guardians.Each sheet is 8" X10" and made out of durable 7.5ml solvent proof Mylar.Ī 8.5" X 11" pre-printed design display poster featuring designs created with this kit is also included. Free for commercial use High Quality Images. 100,000+ Vectors, Stock Photos & PSD files. Anyone using the information provided by Kidadl does so at their own risk and we can not accept liability if things go wrong.ģ) Because we are an educational resource, we have quotes and facts about a range of historical and modern figures. Find & Download Free Graphic Resources for Grim Reaper Tattoo. Color in online Print page More Fantasy Free Printable Stencils. Kidadl cannot accept liability for the execution of these ideas, and parental supervision is advised at all times, as safety is paramount. We recommend that these ideas are used as inspiration, that ideas are undertaken with appropriate adult supervision, and that each adult uses their own discretion and knowledge of their children to consider the safety and suitability. Our recommended activities are based on age but these are a guide. water based acrylics and body art tattoo paint. All mylar stencils are durable & reusable that can be used with solvent or water-based base. Suitable for motorcycles, cars, tatoos, cakes, t-shirts, graphic arts, and all types of DIY craft project. We recognise that not all activities and ideas are appropriate for all children and families or in all circumstances. Premium Quality Grim Reaper 4 Airbrush Stencil Template. However, with enough exploration, one can easily curate a. Grim Reaper tattoos typically come in the same form. The tattoo heavily consists of black ink and is extremely painful to wear. The first grim reaper in the tattoo is big, and the second one under its shade. ![]() Some other elements which can be added to the tattoo design to add new meanings to it are the hourglass, a soul, the Pale Horseman and Death’s Handmaiden. ![]() The tattoo consists of two grim reapers along with skulls at the bottom of the tattoo. In some designs, the reaper is shown as a faceless character, with only hollow eyes in the name of facial features, while others may picture the Grim Reaper as a skull or skeleton. The sketch is often draped in a black hood and carries a scythe as its reliable companion. The nightmare reaper tattoo is among the most horrific tattoos you can wear on the shoulder or calf. We will always aim to give you accurate information at the date of publication - however, information does change, so it’s important you do your own research, double-check and make the decision that is right for your family. After skull tattoos, symbols of coffins, and silhouettes of candles, the shadow of a Grim Reaper is what depicts death in the world of tattoos. We also link to other websites, but are not responsible for their content.Ģ) At Kidadl, we strive to recommend the very best activities and events. Please note that Kidadl is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon. Prices are correct and items are available at the time the article was published but we cannot guarantee that on the time of reading. If you purchase using the Buy Now button we may earn a small commission. We hope you love our recommendations for products and services! What we suggest is selected independently by the Kidadl team. ![]() 1) Kidadl is independent and to make our service free to you the reader we are supported by advertising.
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Anime cute sexy hot ecchi girl3/21/2024 It has an uncensored version, OVAs, and the hentai scene for it is huge.The allusions in this anime are great and if you understand them helps you appreciate the writing put into the characters.This anime will have you in the heat every episode with the sheer amount of ecchi in it. ![]() If you’re interested in seeing hot anime chicks that are uncensored, you have got to watch this anime. This is the go-to ecchi anime for all types of viewers, some could even say it’s the first step into becoming an anime degen. Things seem to be looking up for Issei when a beautiful girl asks him out on a date, although she turns out to be a fallen angel who brutally kills him! However, he gets a second chance at life when beautiful senior student Rias Gremory, who is a top-class devil, revives him as her servant, recruiting Issei into the ranks of the school’s Occult Research club. ![]() High school student Issei Hyoudou is your run-of-the-mill pervert who does nothing productive with his life, peeping on women and dreaming of having his own harem one day. The series also won “Best Vocal Ensemble in an Anime Television Series/OVA” in 2015 for season 2 At the Behind the Voice Actors Awards, Jamie Marchi won “Best Female Lead Vocal Performance in an Anime Television Series/OVA” in 2014. High School DxD is known for its award-winning English dub. Famously recognized as a great example of a “soft-hentai”.The main character does have sex with multiple girls over the course of the series, but these scenes have a meaning.The main plot of the anime is presented in a multi-arc branching format that independently tells the stories of Kazuha, Akira, Nao, and Sora while sharing certain common episodes.However Yosuga no Sora is a genuinely enticing drama that, when looked at with an unbiased and thoughtful perspective, displays a heartwarming drama. Meeting both old acquaintances and new companions alike, the story of Haruka and Sora only gets more convoluted as their lives are slowly influenced by different acts of love, friendship, envy-and perhaps even lust.Īt first sight, many people might turn away in disgust when finding out the anime includes incest and softcore, while disregarding all other aspects of the story. However, revisiting such a nostalgic place also means recalling all the memories the two of them made together, be it those that gave them blissful joy or those that made them suffer painful sorrow. Starting their lives anew, they return to their childhood home. ![]() Twins Haruka and Sora Kasugano have lost both of their parents. Originally a visual novel, Yosuga no Sora was adapted into a 12-episode anime that features a 3 minutes omake segment at the end of each episode.
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Comanche language greeting3/20/2024 ![]() Meanwhile, actual Native languages are dying. But we still get offerings like the 2008 TV movie “Comanche Moon,” in which actors alternate a few words of the Comanche language with nonsense syllables, according to Todd McDaniels, a linguist at the Comanche National College. Producers hired a Lakota-language instructor, and although Kevin Costner’s accent is apparently atrocious and there are a number of grammatical errors, it’s a big step beyond “How!” More recently, Terrence Malick’s “The New World” employed a linguist to resurrect a lost form of Virginian Algonquian spoken at the time of the Jamestown settlement. “Dances With Wolves,” from 1990, is generally viewed as mainstream Hollywood’s first attempt to use Native languages in a sensitive way, even if the results are at times groan-inducing. Some films even dubbed English backward in the soundtrack and played it off as an “Indian” language. But plenty relied heavily on Peter Pan-style “Yip yip yip!” yodeling. Actors playing Plains Indians in huge feather headdresses faked versions of Lakota or Sioux to varying degrees of accuracy, or, as the 2008 documentary “Reel Injun” recounts, spoke random off-script dialogue in their own languages. In the ’30s, ’40s, and ’50s, cowboy westerns made the occasional half-hearted effort at realism. ![]() How the filmmakers handle this dilemma reveals much about Hollywood’s uneasy, evolving relationship to the languages spoken here for millennia before English. Tonto (who in the original story was Potawatomi, not Comanche) is a central figure in the Lone Ranger story at the same time, with his pidgin dialect, he represents the lamest stereotype of Hollywood Indian. Kemosabe is just part of the dubious linguistic freight involved in remaking “The Lone Ranger” for 2013. In this summer’s version, Tonto makes his own suggestion: “Wrong brother,” he says, referring to a running joke that the Ranger’s brother would have made a better hero. Theories on kemosabe’s origin have ranged from the name of a summer camp attended by Fran Striker, the writer of the series, to an Indian-ish term for “faithful friend,” to a corruption of the Spanish phrase “quien no sabe”-he who doesn’t understand. In a scene from the new “The Lone Ranger” movie, Armie Hammer’s Lone Ranger turns to his Comanche sidekick, Johnny Depp’s Tonto, and asks a question that’s been bedeviling fans and linguists since the series first came on the radio in 1933: What does kemosabe, Tonto’s nickname for the masked man of justice, actually mean?
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Firewatch ending explained reddit3/20/2024 Overall a great game and it touches on some deem subjects and morals. Even if you ask here to meet up together she seems hesitant about it and making excuses. Thats why she left first instead of meeting you in the helicopter she did not want to make it harder to say goodbye forever. ![]() I think that is the cannon ending because even when you talk to Delilah she seems very hesitant and not wanting to be with you. The hole summer was basically will become just a memory and go you go back to Julia who has Alzheimer to live out the rest of your life knowing things will never be normal. Basically no matter what you end up never meeting Delilah and your forced to go back to things before. It was one of the most depressing endings in any game i have played and maybe even experienced any movie or book. Because of these inconsistencies I was waiting for some reveal that the people behind the conspiracy had faked the whole ned faking the conspiracy thing and Henry just buys it.Īnd the real sad thing is I totally prefer the ending we got as far as a story goes, I like that it wasn't some weird conspiracy but it just doesn't work for me with all these inconsistencies. That's what "ruins" the ending for me that whole research station section, so I'm almost hoping someone comes along and tells me I missed something that explains it. Just her superior, I guess?įor me I agree that the ending kind of falls flat, not really for the same reasons stated here, my problem with the ending isn't that it was a red herring, my problem with the ending is it not adding up(OR I'm an idiot and missed dialogue/picked it up wrong), this guy(ned) is in the woods for 3 years and somehow sets up that elaborate ruse(in a crazy short amount of time, where did all of the fancy equipment come from, surely I'm not supposed to believe he made that, is it supposed to be implied that Delilah didn't know that there was a research station over an acre in size inside this park.lets say that is true and she just didn't know it's there, am I supposed to believe that expensive equipment is left in a tent behind a measly 10ft fence. ![]() I didn't bring it up to her thinking I'd learn later, but it never happened. The game actually makes a point with that.Īnyway, it's my rambling opinion just after finishing it.Īlso: anybody has any idea who Delilah is talking to at the very beginning of the game. I disagree with people saying that Henry and Delilah should end up with each other or that the relationship would've made sense if she waited for him. It stumbles a bit, but I think there is a strong core in there. It feels as if the writers weren't confident enough in the story they had about normal people, whereas thinking back to it, the moments where you play before the starts of the mystery, when you just start out your relationship with Delilah, seem to be the best in retrospect. The reveal there (who is spying on you) is thematically relevant (Ned, as Henry and Delilah, tried to run away from its past) but shows how the set-up is just a trick and a gimmick. ![]() I think the ending and story with Delilah is actually great, my problem is how it tries to fool you with its thriller part. So why, in a Game, do we need to contextualize that story within a grand, horrifying mystery? Don't make a story about figuring out the mystery of two disappeared teens and a mad-man secretly tracking your every move, just so you can gently slip in the actual focus of your game.įilms can tell real-world stories about real-world people, without pretending it's secretly an action-horror-murder-mystery to keep people interested. It's the same issue I had with Gone Home I think it's kind of immature to treat people who play games like idiots who need to be 'fooled' into thinking there's something exciting or scary around the corner, and that's really how it comes off when you spend most of your narrative clearly alluding to a grander conspiracy only to say "Nah it was just regular people all along!" It's a cheap trick, and I dislike the fact that it's been used twice now - If you want a story about the relationship between Delilah and Henry, make that game. I loved 95% of the game, but I completely agree that ending isn't perfect, and I don't at all buy the "Well it was always a simple story about regular people" explanation. I'll copy and paste my thoughts from Reddit thread talking about it being kind of a disappointment: ![]() So how did you guys feel about that ending, huh? Was it a perfect subversion of the mystery narrative, or a disappointing anti-climax that undermined its own plot? Speak out!
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Cuphead multiplayer online ps43/20/2024 ![]() ![]() Play as Cuphead or Mugman (in single player or local co-op) as you traverse strange worlds, acquire new weapons, learn powerful super moves, and discover hidden secrets while you try to pay your debt back to the devil!Īnother helping of classic Cuphead action awaits you in Cuphead – The Delicious Last Course! Brothers Cuphead and Mugman are joined by the clever, adventurous Ms. traditional hand drawn cel animation, watercolor backgrounds, and original jazz recordings. Inspired by cartoons of the 1930s, the visuals and audio are painstakingly created with the same techniques of the era, i.e. virtual) required for installing this repackĬuphead is a classic run and gun action game heavily focused on boss battles. Repack uses XTool library by Razor12911.Language can be changed in game settings.HDD space after installation: up to 6 GB.After-install integrity check so you could make sure that everything installed properly. ![]() Installation takes 3-11 minutes (depending on your system and selected components).Significantly smaller archive size (compressed from cumulative 5.7 to 3.7/4.1 GB).Selective Download feature: you may skip downloading and installing of bonus OSTs.100% Lossless & MD5 Perfect: all files are identical to originals after installation.Two missing bonus OSTs (original game + DLC, 586 MB) added, thanks to GOG and nothingmore!.Game version: v1.3.2, The Delicious Last Course DLC is included and activated.Incomplete SKIDROW crack replaced with CODEX one, along with Goldberg crack/emu available in NoDVD folder after installation.Based on .Course-SKIDROW ISO release: sr-cupheadthedeliciouslastcourse.iso (5,463,709,696 bytes).Filehoster: OneDrive (Uploaded by DyR0 t(-_-t), compatible with torrent mirrors)ĭiscussion and (possible) future updates on CS.RIN.RU thread Screenshots (Click to enlarge).Filehoster: MultiUpload (10+ hosters, interchangeable).Genres/Tags: Arcade, Run and gun, Side, 2D #777 Updated Cuphead v1.3.2 + DLC + Bonus OSTs
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Cat tortoiseshell calico3/20/2024 ![]() The three-colored coloration brings to felines the sense of freedom, independence, and smartness. It is only a coloration type that can be met in various litters.Īs the brown, black, or blue tortoiseshell cat, a calico has its own features of character. But note that calicos are not the separate-standing breed. This list is rather long to mention all the buddies at once. Do not forget that the third color in the three-component mix is always white!Īmong calico cat breeds are Maine Coons, Persians, Ragdolls, Cornish Rex, etc. Dense calico cat colors are usually black, dark grey, orange, and deep brown. All the colors are light and far from bright or intensive ones. A dilute calico cat can impress with its gorgeous look. It is worth noting that there are dense and dilute coloration types to take into account. For example, you can see the happy blend of white, lilac, and blue or cream, brown, and white as well. In comparison with torts, calico-friendly felines always obtain white color on their coat.Ĭalico kittens are three-colored bewhiskered buddies with very interesting pattern combinations. Adopt a tortoiseshell feline to have a lucky four-leaf clover at home! Overview of CalicoĪ tender coloration that contains light harmonious patches and spots on the fur is about calicos.
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Best penthouse magazine3/20/2024 ![]() Penthouse began publication in 1965 in the United Kingdom and in North America in 1969, an attempt to compete with Hugh Hefner's Playboy. He occasionally created cartoons for Bill Box's humorous greeting card company Box Cards. To support his family, Guccione managed a chain of laundromats until he got work as a cartoonist on an American weekly newspaper, The London American, while Muriel started a business selling pinup posters. He eventually met an English woman, Muriel Hudson, moved to London with her, and married her. The marriage failed, and he left his wife and child to go to Europe to be a painter. The couple had a daughter, Tonina (1949-2020). In his teens, Guccione married his first wife, Lilyann Becker. He attended high school at Blair Academy, a prep school in Blairstown, New Jersey. An altar boy, he considered but rejected entering the priesthood. Guccione was born in Brooklyn, New York, of Sicilian descent and raised Catholic in Bergenfield, New Jersey, the eldest child of Anthony, an accountant, and Nina, a housewife. In 2003, Guccione's publishers filed for bankruptcy and he resigned as chairman. However, he made some extravagant investments that failed, and the growth of free online pornography in the 1990s greatly diminished his market. By 1982 Guccione was listed in the Forbes 400 wealth list, and owned one of the biggest mansions in Manhattan. ![]() This was aimed at competing with Hugh Hefner's Playboy, but with more explicit erotic content, a special style of soft-focus photography, and in-depth reporting of government corruption scandals and the art world. He founded the adult magazine Penthouse in 1965. ![]() Robert Charles Joseph Edward Sabatini Guccione ( / ɡ uː tʃ i ˈ oʊ n i/ goo-chee- OH-nee Decem– October 20, 2010) was an American photographer and publisher.
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Multicolor stack in imagej fiji3/20/2024 ![]() ![]() But you can use the Watershed tool to detect and separate these objects so they can be counted as individual objects again. This can be a problem for some types of analysis, such as counting. When you create a mask/binary of these they will appear as a single object. Sometimes separate objects in your image will be touching. Erode will remove a thin layer (1 pixel) from around the edge of the mask/binary. Dilate will add a thin layer (1 pixel) around the edge of the existing mask/binary. To add or remove from the edges of a mask or binary you can use the Dilate or Erode tools. This could be useful for things like membrane measurements or morphology analysis. Sometimes you will need to add or remove a small layer from the edges of the mask/binary. Small gaps left in the mask or binary after thresholding can easily be corrected for by using the Close tool. All of these tools can be found under Process -> Binary. There are a number of tools available to help you get the best fit to your data. Sometimes adjustments need to be made to binaries/masks before analysis to ensure it is accurately representing the data. Macs will often produce reversed background and foreground colours also. However, depending on your settings (both the general settings in FIJI, and those selected from the binary options above), the background and foreground colours can somtimes be reversed in the mask and binary. Generally masks and binaries will appear as shown in the images below. To generate a binary go to Process -> Binary -> Make Binary.įor this option, a window will open with binary options, select your options and click OK to generate the binary image. You can also go to Process -> Binary -> Convert to Mask. As shown above, if you select Apply in the threshold window it will convert the threshold to a mask. If you need to convert your threshold to a mask, or binary, there are several options you can use. The list in the log that was created will show the order of the algorithms used in the montage.įrom this montage you can decide which algorthim may suit your data the best and apply it using the steps above. Press OK.Ī montage will be generated showing what each auto threshold algorithm would produce on the image. In the dialog box that opens up set the Method to Try All, make sure the White objects on black background (for fluorescence images) and Show threshold values in log window boxes are ticked. To use the auto-test for thresholds, go to Image -> Adjust -> Auto Threshold. You can also automatically test the range of threshold algorithms on your image without scrolling through the dropdown list one at a time. ( Note: there will be more details on masks and binaries in the next sub-section). When you are happy with the selection (ie: the red covers the parts of the image you want selected well, without any additional background), close the threshold box to keep is as a threshold only (A in the image below), or click Apply (B in the image below) then close the threshold box to convert it to a mask. Reset will remove any overlayed threshold and allow you to begin again. Rather than manually adjust, you can also select your algorithm and use the Auto button to allow the program to adjust the threshold levels for you. The red box on the histogram shows which parts of the histogram are being thresholded. The top slider sets the bottom range of the threshold and the bottom slider adjusts the top range of the threshold. Choose the threshold that looks liek the best fit for your data then further adjust by moving the sliders above. The drop down box on the left (the one that says 'Default' in the image above) has 16 different auto threshold algorithms to choose from. In the window that opens, ensure the box beside Dark background is checked for fluorescence images. To apply a threshold, select your image and go to Image -> Adjust -> Threshold (or shortcut Ctrl+Shift+T).Ī red overlay will be placed on the parts of the image that are selected by the threshold and a window will open where you can adjust the threshold on the image. Threshold Imageįor a number of basic measurements an image first needs to have a threshold applied to it. The image Nuclei-1.tif and the image set ‘ RGBstackProjection, RGB-blue, RGB-green and RGB-red’ are used for demonstration purposes in this section. This section outlines the various options for masks and filters and how to apply them to your images prior to analysis. Creating thresholds and masks and applying filters forms the basis for a number of analysis methods. |